Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

p53 alterations in thymic epithelial tumours

G Weirich1, P Schneider, C Fellbaum

  • 1Institute of Pathology, Technical University of Munich, School of Medicine, Germany.

Virchows Archiv : an International Journal of Pathology
|July 1, 1997
PubMed
Summary

Identifying p53 alterations in thymic epithelial tumours using immunohistochemistry offers a new prognostic factor. This molecular marker aids in predicting tumour behavior beyond traditional histopathology, improving patient outcome evaluation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Interdisciplinary ultrasound-guided, minimally invasive autopsy in COVID-19-deceased patients in the intensive care unit of a university hospital : A proof-of-concept study].

Pathologie (Heidelberg, Germany)·2023
Same author

Fluorescence imaging of invasive head and neck carcinoma cells with integrin αvβ6-targeting RGD-peptides: an approach to a fluorescence-assisted intraoperative cytological assessment of bony resection margins.

The British journal of oral & maxillofacial surgery·2018
Same author

Primary skin manifestation of plasmoblastic lymphoma in an AIDS patient with long-term survival.

Journal of the European Academy of Dermatology and Venereology : JEADV·2017
Same author

Distribution of tritiated ecdysone in salivary glands and other tissues ofRhynchosciara andChironomus larvae an autoradiographic study.

Wilhelm Roux' Archiv fur Entwicklungsmechanik der Organismen·2017
Same author

Intraoperative cell isolation for a cytological assessment of bone resection margins in patients with head and neck cancer.

The British journal of oral & maxillofacial surgery·2017
Same author

[Blast-like tumor tissue in cervical lymph node with expression of placental alkaline phosphatase].

Der Pathologe·2017

Area of Science:

  • Oncology
  • Molecular Pathology
  • Cancer Genetics

Background:

  • Prognosis of thymic epithelial tumours (TETs) is challenging due to limitations in predicting malignant behavior solely by histopathology.
  • There is a need for molecular markers to enhance the accuracy of TET prognostication.

Purpose of the Study:

  • To identify a reliable molecular marker for evaluating thymic epithelial tumours.
  • To assess the utility of p53 alterations as a prognostic factor in TETs.

Main Methods:

  • Analysis of 44 TETs for p53 alterations using immunohistochemistry (IHC) with antibodies D0-1 and CM-1.
  • Employing PCR-based single-strand conformation polymorphism and DNA sequencing for p53 gene mutation analysis.

Main Results:

Related Experiment Videos

  • Three distinct tumour groups emerged: non-organotypic thymic carcinomas (frequent p53 alterations/mutations), malignant thymomas (frequent p53 alterations, no mutations), and benign thymomas (rare p53 alterations, no mutations).
  • IHC p53 detection correlated significantly with clinical stage (P < 0.01), pathohistology (P < 0.01), and survival times (P < 0.05).
  • Differences in p53 detection between tumour types suggest specific alterations in thymomas.

Conclusions:

  • Immunohistochemical detection of p53 is a valuable prognostic tool for thymic epithelial tumours.
  • p53 analysis provides prognostic information that complements histopathological evaluation.
  • This molecular approach can improve the clinical management and outcome prediction for patients with TETs.