The effect of burn blister fluid on fibroblast contraction

Burns of the hand seem to be followed by more stiffness and contractures than other injuries. This study was aimed at examining whether burn exudate contributes to this difference. Five cell strains of human dermal fibroblasts were each treated with three samples of burn blister fluid and the effect compared with the rate of contraction of free floating fibroblast populated collagen lattices (FPCL). In addition, the response of fibroblasts from the dermis, fascia and peritendinous sheath of the hand to burn fluid were compared both in FPCLs and by the tensile forces they generated in the culture force monitor. Results of this study consistently show that the addition of burn fluid to culture medium stimulates fibroblasts to contract to a greater degree in the first 24-48 h. It is proposed that the presence of burn blister fluid provides materials which promote contraction. This in turn may contribute to the postburn stiffness seen clinically.