Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sulfide influence on polymorphonuclear functions: a possible role for Ca2+ involvement

M A Mariggiò1, F Pettini, R Fumarulo

  • 1Department of Biomedical Sciences and Human Oncology, Faculty of Medicine, University of Bari, Italy.

Immunopharmacology and Immunotoxicology
|August 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correlation between occlusal trauma and oral microbiota: a microbiological investigation.

Journal of biological regulators and homeostatic agents·2021
Same author

Gender medicine: the impact of probiotics on male patients.

La Clinica terapeutica·2020
Same author

The pivotal role of oral microbiota in health and disease.

Journal of biological regulators and homeostatic agents·2020
Same author

Effects of sea salt rinses on subjects undergone to oral surgery: a single blinded randomized controlled trial.

La Clinica terapeutica·2019
Same author

Effect of activated charcoal probiotic toothpaste containing <i>Lactobacillus paracasei</i> and xylitol on dental caries: a randomized and controlled clinical trial.

Journal of biological regulators and homeostatic agents·2019
Same author

A pilot study of human mesenchymal stem cells from visceral and sub-cutaneous fat tissue and their differentiation to osteogenic phenotype.

European review for medical and pharmacological sciences·2019

Sulfide, a bacterial toxin, impairs polymorphonuclear cell (PMN) functions like chemotaxis but enhances their oxidative burst. This suggests a complex role in periodontal defense, potentially aiding bacterial killing in anaerobic environments.

Area of Science:

  • Immunology
  • Microbiology
  • Periodontology

Background:

  • Polymorphonuclear cells (PMN) are crucial for oral host defense against bacteria.
  • Bacterial virulence factors can modulate PMN phagocytic activity.
  • Sulfide is a toxic metabolite produced by oral pathogens.

Purpose of the Study:

  • To investigate the effect of sulfide on key PMN functions: chemotaxis, degranulation, and oxidative burst.
  • To understand how sulfide influences calcium-dependent and independent PMN responses.

Main Methods:

  • Exposure of PMN to sodium sulfide (H2S source) at varying concentrations.
  • Assessment of PMN chemotaxis and degranulation (azurophilic and specific granule release) induced by FMLP and PMA.
  • Measurement of cytosolic free Ca2+ concentration ([Ca2+]i) changes.

Related Experiment Videos

Main Results:

  • Sulfide depressed calcium-dependent PMN chemotaxis and azurophilic granule release.
  • Sulfide did not affect calcium-independent specific granule release.
  • Sulfide inhibited ionomycin-induced cytosolic free Ca2+ increase.
  • Hydrogen sulfide primed PMN for an enhanced oxidative burst response.

Conclusions:

  • Sulfide impairs crucial calcium-dependent PMN functions, potentially compromising host defense.
  • Sulfide enhances PMN oxidative burst, which may contribute to bacterial killing in reoxygenated anaerobic sites.