Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solid-phase extraction in amphetamine and methamphetamine analysis of urine

M R Lee1, S C Yu, C L Lin

  • 1Department of Chemistry National Chung-Hsing University, Taichung, Taiwan (R.O.C.).

Journal of Analytical Toxicology
|July 1, 1997
PubMed
Summary

This study optimized solid-phase extraction for detecting methamphetamine and amphetamine in urine. A C8-SCX mixed adsorbent achieved over 86% recovery, crucial for forensic toxicology.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Construction of TRAF6 ubiquitin site 331 mutant colorectal cancer cell stable line and its effect on biological behavior of colorectal cancer cells].

Zhonghua zhong liu za zhi [Chinese journal of oncology]·2023
Same author

Comparison of micellar casein isolate and nonfat dry milk for use in the production of high-protein cultured milk products.

Journal of dairy science·2022
Same author

Molecular Relationships in Biofilm Formation and the Biosynthesis of Exoproducts in Pseudoalteromonas spp.

Marine biotechnology (New York, N.Y.)·2022
Same author

Labeled oxytocin administered via the intranasal route reaches the brain in rhesus macaques.

Nature communications·2020
Same author

Tunability of Acoustic and Mechanical Behaviors in Breast Tissue Mimicking Materials.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2020
Same author

Ultrasonic drilling for the characterisation of building stones and salt induced decay.

Ultrasonics·2019

Area of Science:

  • Forensic Chemistry
  • Analytical Chemistry

Background:

  • Accurate detection of methamphetamine and amphetamine in biological samples is vital for forensic toxicology.
  • Solid-phase extraction (SPE) is a common sample preparation technique for drug analysis.

Purpose of the Study:

  • To systematically compare the recovery rates of methamphetamine and amphetamine from spiked urine using various solid-phase extraction (SPE) methods.
  • To optimize SPE conditions, including sorbent type, pH, solvent composition, and urine concentration, for enhanced drug recovery.

Main Methods:

  • Evaluated four SPE procedures: C8, C18, strong cation exchanger (SCX), and a mixed C8-SCX adsorbent.
  • Investigated the impact of sample pH, solvent composition, and urine matrix concentration on extraction efficiency.
  • Utilized gas chromatography-mass spectrometry (GC-MS) in electron impact mode for drug detection and quantification.

Related Experiment Videos

Main Results:

  • The C8-SCX mixed adsorbent yielded the highest recoveries: >86% for methamphetamine and >88% for amphetamine.
  • Optimal elution was achieved with a mixed solvent (CH2Cl2-i-propanol-NH4OH, 78:20:2) at pH 6.
  • Urine matrix concentration did not significantly affect extraction efficiency.
  • Detection limits were 0.03 µg/mL for methamphetamine and 0.07 µg/mL for amphetamine.

Conclusions:

  • The C8-SCX mixed SPE method provides superior recovery for methamphetamine and amphetamine in urine compared to single sorbent methods.
  • Optimized SPE conditions enhance the sensitivity and reliability of GC-MS analysis for these drugs in forensic applications.
  • This optimized method is suitable for routine toxicological screening and quantitative analysis of amphetamines in urine samples.