Automated analysis of thiamine, ascorbic acid, and vitamin A

Methods currently used in most nutrient analysis laboratories are the classical AOAC methods. One of the major challenges facing the analytical chemist is the need for new or modified quantitative and reliable nutrient assay methods, which are rapid and less labor intensive. Recently, numerous vitamin assays have been published which use continuous flow and high pressure liquid chromatographic techniques. This paper deals with some of the recent methods reported for analysis of thiamine and ascorbic acid, using continuous flow wet chemistry, and high pressure liquid chromatography for vitamin A in food products.