Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multidimensional chromatography coupled with mass spectrometry for target-based screening

Y F Hsieh1, N Gordon, F Regnier

  • 1PerSeptive Biosystems Inc., Framingham, MA 01701, USA.

Molecular Diversity
|April 1, 1997
PubMed
Summary

Automated multidimensional chromatography coupled with mass spectrometry (Selectronics) enables rapid identification of high-affinity drug lead compounds. This advanced screening technique accelerates drug discovery by efficiently selecting and characterizing potential drug candidates from diverse chemical libraries.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identifying genetic loci associated with an increased risk for early-onset colorectal cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2024
Same author

Nitrogen and fatty acid rumen metabolism in cattle offered high or low polyphenol oxidase red clover silage.

Animal : an international journal of animal bioscience·2018
Same author

Automated circuit fabrication and direct characterization of carbon nanotube vibrations.

Nature communications·2016
Same author

Hemostatic effects of two desogestrel-containing combined oral contraceptive regimens: a multinational, multicenter, randomized, open-label study.

Clinical and experimental obstetrics & gynecology·2016
Same author

Anti-Cancer Treatments in Elderly (≥75 Years Old) Patients: A Retrospective Analysis.

Value in health : the journal of the International Society for Pharmacoeconomics and Outcomes Research·2016
Same author

Improved upper limits on the stochastic gravitational-wave background from 2009-2010 LIGO and Virgo data.

Physical review letters·2014

Area of Science:

  • Drug Discovery and Development
  • Analytical Chemistry
  • Biochemistry

Background:

  • Recent advances in solid-phase synthesis and automated screening have transformed drug discovery.
  • Molecular diversity strategies necessitate efficient, automated methods for selecting lead candidates.
  • Traditional methods for identifying and characterizing lead compounds can be time-consuming.

Purpose of the Study:

  • To demonstrate an automated target-based screening technique for identifying high-affinity binders.
  • To showcase the utility of multidimensional automated chromatography coupled to electrospray ionization mass spectrometry (Selectronics) in drug discovery.
  • To reduce the time and effort required for lead compound identification and characterization.

Main Methods:

  • Utilized multidimensional automated chromatographic techniques coupled to electrospray ionization mass spectrometry (Selectronics).

Related Experiment Videos

  • Employed target-based selection by capturing tightly bound affinity leads by target biomolecules.
  • Applied high-resolution separation with sensitive detection for subsequent release and analysis.
  • Screened several organic and natural compound libraries.

    • Main Results:

    • Successfully demonstrated an automated target-based screening technique.
    • Identified high-affinity binders as potential lead compounds from diverse libraries.
    • Provided maximum characterization information in a single screening run.
    • Significantly reduced the time required for lead compound identification and characterization.

    Conclusions:

    • Automated multidimensional chromatography with mass spectrometry (Selectronics) is an effective approach for accelerated drug discovery.
    • This technique facilitates the rapid selection and characterization of high-affinity lead compounds.
    • Selectronics offers a powerful tool for molecular diversity strategies and lead optimization.