Acetylcholine receptor of a myogenic cell line, L6

The biochemical characters of acetylcholine receptor (AChR) in a myogenic cell line, L6, are as follows. 1. AChR extracted with Triton X-100 has a molecular weight of 5.5 X 10(5) determined by gel-chromatography, an apparent S value of 9.7 determined by sucrose density gradient centrifugation, and an isoelectric point of 5.3. AChR is shown to interact with concanavalin A. 2. The association rate constant of the binding reaction between AChR and alpha-bungarotoxin (alpha-BGT) labelled with 125I is 1 X 10(6) M-1 with S-1 at 35 degrees C. Preincubation with d-tubocurarine blocks the binding. The half-life of the AChR-toxin complex exceeds 180 h.