Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intestinal stem cell division and genetic diversity. A computer and experimental analysis

J L Tsao1, S D Davis, S M Baker

  • 1Department of Pathology, University of Southern California School of Medicine, Los Angeles 90033, USA.

The American Journal of Pathology
|August 1, 1997
PubMed
Summary

Somatic mutations increase with age, accelerated in mice lacking the DNA mismatch repair gene Pms2. This study quantizes mutation rates in normal intestinal cells, supporting microsatellite loci as molecular tumor clocks.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial Expression of Concern: Adenomatous polyposis coli (APC) regulates miR17-92 cluster through β-catenin pathway in colorectal cancer.

Oncogene·2024
Same author

The association between gut microbiome and growth in infants with cystic fibrosis.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2023
Same author

Comparison of traditional two-injection dorsal digital block versus transthecal and subcutaneous single-injection digital block: A systematic review and meta-analysis.

Hand surgery & rehabilitation·2021
Same author

Phylogenetic and phylodynamic analyses of hepatitis C virus subtype 1a in Okinawa, Japan.

Journal of viral hepatitis·2018
Same author

Effects of Hypoxia and Anoxia on Larval Settlement, Juvenile Growth, and Juvenile Survival of the Oyster Crassostrea virginica.

The Biological bulletin·2018
Same author

Hemoglobin Structure and Function in the Rat-Tailed Sea Cucumber, Paracaudina chilensis.

The Biological bulletin·2018

Area of Science:

  • Genetics
  • Molecular Biology
  • Cancer Research

Background:

  • Somatic mutations accumulate with age.
  • DNA mismatch repair (MMR) deficiency accelerates mutation rates.
  • The Pms2 gene is crucial for MMR.

Purpose of the Study:

  • To investigate somatic mutation accumulation in normal intestinal epithelium of Pms2-deficient mice.
  • To quantify mutation rates and understand their patterns in the context of intestinal renewal.
  • To assess the utility of microsatellite loci as molecular tumor clocks.

Main Methods:

  • Analysis of microsatellite allele distributions in Pms2-/- mouse intestinal tissues.
  • Age-based comparison of genetic diversity and mutation patterns.
  • Computer simulations to model mutation rates, cell death, and population size.

Related Experiment Videos

Main Results:

  • Increased genetic diversity and mutation accumulation with age were observed.
  • Complex mutation patterns and variances were found within individual mice.
  • Simulations supported mutation rates of 0.0020-0.0025 mutations/division, nonrandom cell death, and small effective cell populations (≤20).

Conclusions:

  • Normal intestinal epithelium exhibits relatively high mutation rates, especially in MMR-deficient states.
  • Small stem cell populations in intestinal renewal exacerbate stochastic mutation accumulation.
  • Findings support microsatellite loci as reliable molecular tumor clocks for analyzing mutation burdens.