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Related Experiment Videos

A consensus sequence for a functional human endogenous retrovirus K (HERV-K) dUTPase

J M Harris1, R H Haynes, E M McIntosh

  • 1Department of Biochemistry, University of Queensland, St. Lucia, Australia.

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
|January 1, 1997
PubMed
Summary

Human endogenous retrovirus K (HERV-K) encodes a dUTPase distinct from the human cellular enzyme. This study clarifies the wild-type HERV-K dUTPase sequence, revealing previous data represented a mutated form.

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Area of Science:

  • Virology
  • Molecular Biology
  • Biochemistry

Background:

  • Human endogenous retrovirus K (HERV-K) is a defective multicopy virus vertically transmitted in humans.
  • HERV-K encodes a potential dUTPase gene, distinct from the human cellular dUTPase.
  • dUTPases possess five conserved active site motifs; previous HERV-K dUTPase sequences showed deviations.

Purpose of the Study:

  • To investigate the accuracy of previously reported HERV-K dUTPase sequences.
  • To determine the wild-type sequence of the HERV-K dUTPase gene.
  • To characterize the functional properties of the wild-type HERV-K dUTPase.

Main Methods:

  • Cloning and sequencing of 22 HERV-K dUTPase genes from human DNA.
  • Construction and expression of a wild-type HERV-K dUTPase gene in Escherichia coli.

Related Experiment Videos

  • Biochemical characterization of the expressed HERV-K dUTPase enzyme.

    • Main Results:

    • Sequencing of 22 HERV-K dUTPase genes revealed variations, with the HERV-K10 sequence representing a mutated form.
    • A wild-type HERV-K dUTPase sequence was reconstructed.
    • The expressed wild-type enzyme exhibited properties similar to dUTPases from other sources.

    Conclusions:

    • The previously reported HERV-K dUTPase sequence was indeed mutated.
    • A functional, wild-type HERV-K dUTPase enzyme has been characterized.
    • The study provides insights into the potential role of HERV-K dUTPase in human disease.