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Cell-to-cell communication and myogenesis

N Kalderon, M L Epstein, N B Gilula

    The Journal of Cell Biology
    |December 1, 1977
    PubMed
    Summary
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    Prefusion chick embryo myoblasts communicate via gap junctions, ionic, and metabolic coupling. This cell communication is not sufficient to induce myoblast fusion, as shown in fusion-arrested cultures.

    Area of Science:

    • Cell Biology
    • Developmental Biology
    • Muscle Physiology

    Background:

    • Cell-to-cell communication is crucial for tissue development.
    • Myogenesis, the process of muscle formation, involves myoblast differentiation and fusion.
    • Gap junctions, ionic, and metabolic coupling are known modes of intercellular communication.

    Purpose of the Study:

    • To characterize cell-to-cell communication in prefusion chick embryo myoblasts.
    • To investigate the biological relevance of myoblast communication in embryonic muscle.
    • To determine if cell communication is a prerequisite for myoblast fusion.

    Main Methods:

    • Prefusion chick embryo myoblast cultures were utilized.
    • Gap junctional, ionic, and metabolic coupling were assessed.

    Related Experiment Videos

  • Myoblast fusion was inhibited using ethyleneglycolbis-(beta-aminoethyl ether)N,N'-tetraacetic acid (EGTA), 5-bromodeoxyuridine (BUdR), and cycloheximide (CHX).

    • Main Results:

    • Prefusion myoblasts communicate through gap junctions, ionic coupling, and metabolic coupling.
    • Gap junctions were observed between myoblasts in embryonic muscle, confirming biological relevance.
    • Cell communication, including gap junctions and ionic coupling, persisted in fusion-arrested myoblast cultures.

    Conclusions:

    • Cell-to-cell communication is present in prefusion myoblasts.
    • Myoblast communication is not a sufficient factor to induce myoblast fusion.
    • Further investigation is needed to fully elucidate the role of cell communication in myogenesis.