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Related Experiment Videos

Modified Lombard-Dowell broth as a general growth medium

M T Jessee, P J Robinson

    Journal of Clinical Microbiology
    |November 1, 1977
    PubMed
    Summary

    A new modified Lombard-Dowell broth effectively supports aerobic and anaerobic bacterial growth, showing comparable results to chopped-meat glucose broth, with a notable exception for Fusobacterium necrophorum.

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    Area of Science:

    • Microbiology
    • Bacteriology

    Background:

    • Accurate cultivation of both aerobic and anaerobic bacteria is crucial for clinical diagnostics.
    • Traditional culture media may have limitations in supporting the growth of diverse bacterial species.

    Purpose of the Study:

    • To evaluate the efficacy of a new liquid medium, modified Lombard-Dowell broth, for culturing aerobic and anaerobic bacteria.
    • To compare its performance against a standard prereduced chopped-meat glucose broth.

    Main Methods:

    • Inoculation of modified Lombard-Dowell broth and chopped-meat glucose broth with aerobic and anaerobic bacterial stock cultures and mixed cultures.
    • Subculturing to aerobic and anaerobic blood agar plates at 48 and 72 hours.
    • Comparison of colony counts and primary plate isolates over an 11-month period.

    Main Results:

    • Modified Lombard-Dowell broth demonstrated comparable growth to chopped-meat glucose broth for most bacterial species.
    • A single exception was observed, where Fusobacterium necrophorum failed to grow in the chopped-meat glucose broth.
    • Overall agreement was high between the two broth systems when assessing isolates from subcultures and primary plates.

    Conclusions:

    • Modified Lombard-Dowell broth is a viable alternative for the simultaneous culture of aerobic and anaerobic bacteria.
    • This new medium shows broad applicability in clinical microbiology, potentially improving detection rates for certain fastidious anaerobes like Fusobacterium necrophorum.

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