Determination of eltanolone in human plasma by high-performance liquid chromatography
D J Jones1, A R Bjorksten, D P Crankshaw
1Department of Anaesthesia, Royal Melbourne Hospital, Victoria, Australia.
Abstract:
A high-performance liquid chromatographic method for the determination of eltanolone in plasma has been developed. Plasma samples containing eltanolone were diluted with acetonitrile to precipitate plasma proteins, and derivatized with 2,4-dinitrophenylhydrazine before direct injection onto a C18 column. The mobile phase was acetonitrile-water (70:30, v/v) containing 0.1% trifluoroacetic acid and detection was by UV absorbance at 367 nm. The quantitation limit was 0.020 microg/ml. The method has proven to be rapid, precise and sensitive in the range of concentrations found during and following intravenous anaesthesia.
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