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Identification and characterization of the gyrB gene from Treponema pallidum subsp. pallidum
L V Stamm1, S R Greene, N Y Barnes
1Department of Epidemiology, School of Public Health, University of North Carolina, Chapel Hill 27599-7400, USA. istamm@email.unc.edu
Abstract:
The nucleotide sequence of a DNA gyrase B subunit gene (gyrB) from Treponema pallidum has been determined. Southern blot analysis of T. pallidum chromosomal DNA indicated that this gene is present as a single copy. The organization of genes flanking the gyrB gene is unique in comparison to that of other bacteria. The gyrB gene encodes a 637 amino acid protein whose deduced sequence has a high degree of homology with type-II topoisomerase ATPase subunits (GyrB and ParE). Five type-II topoisomerase motifs, an ATP-binding site (Walker A), and amino acid residues that putatively interact with ATP, are highly conserved in the T. pallidum GyrB protein.