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Disaccharide analysis of skin glycosaminoglycan in localized scleroderma
Y Yokoyama1, O Ishikawa, Y Miyachi
1Department of Dermatology, Gunma University School of Medicine, Maebashi, Japan.
Background:
Altered metabolism of skin glycosaminoglycan has rarely been investigated in localized scleroderma in contrast to systemic sclerosis.
Objective:
The aim of this investigation is to elucidate the change in skin glycosaminoglycan of localized scleroderma.
Methods:
We analyzed 5 skin samples of localized scleroderma and 10 site-matched control skin samples using high performance liquid chromatography after 1-phenyl-3-methyl-5-pyrasolone labeling.
Results:
The involved skin constantly showed an increased amount of delta Di-4S(DS), the main disaccharide unit of dermatan sulfate, a decreased amount of delta Di-HA, the main disaccharide unit of hyaluronic acid, and an elevated ratio of delta Di-4S(DS)/delta Di-HA as compared with the uninvolved skin or the site matched control skin.
Conclusion:
These results correlate well with those findings in systemic sclerosis, indicating that the alteration in skin GAG may be closely related to the fibrotic process.