Methods for the determination of mutagenic heterocyclic amines and their applications in environmental analysis

The environmental analysis of heterocyclic amines is important to preserve human health, because all these compounds are potent mutagens and many are carcinogens in experimental animals. This review summarizes the analytical methods for the determination of mutagenic heterocyclic amines and their applications to various environmental samples. High-performance liquid chromatographic methods with ultraviolet, electrochemical and fluorescence detections are commonly used for the quantification of heterocyclic amines. Moreover, liquid chromatography-mass spectrometry, gas chromatography, gas chromatography-mass spectrometry, capillary zone electrophoresis and enzyme-linked immunosorbent assay are also developed. Heterocyclic amines in complex environmental matrices are present at low parts per billion or less, so that the sample preparation influences the reliable and accurate analysis of these compounds. Mutagenic heterocyclic amines are widely distributed in a number of ambient environmental components such as airbone particles, diesel-exhaust particles, cigarette smoke, cooking fumes, rain water, sewage water, incineration-ash and soil. These facts suggest that mutagenic heterocyclic amines are likely to be ubiquitous environmental pollutants and humans are continually exposed to these compounds in normal daily life.