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Calibration of micropipets using the bioluminescent protein aequorin
A L Grosvenor1, C L Crofcheck, K W Anderson
1Department of Chemical and Materials Engineering, University of Kentucky, Lexington 40506-0055, USA.
Analytical Chemistry
|August 1, 1997
Abstract:
A new method for calibrating micropipets and determining accurate injection volumes using a pressure-based injector has been developed. This method employs the bioluminescent protein aequorin and can be used to determine injection volumes as small as 3 pL. The calibration plots are linear over at least 3 orders of magnitude. In contrast to conventional micropipet calibration methods that employ fluorescent molecules, the present method produces small background signals.