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Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
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Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

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Cytogenetic analysis of Liza ramada (Pisces, Perciformes) by different staining techniques and fluorescent in situ

A R Rossi1, E Gornung, D Crosetti

  • 1Department of Animal and Human Biology, University of Rome, Italy.

Heredity
|July 1, 1997
PubMed

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...