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X-ray- and ultraviolet-radiation-induced mutations in Muta mouse
1Department of Radiation Research, Tohoku University School of Medicine, Sendai, Japan.
Radiation Research
|August 1, 1997
Summary
The Muta mouse model shows increased mutation frequencies after X-ray or UV radiation exposure. However, this model is less sensitive to X-ray-induced mutations compared to UV-induced mutations.
Area of Science:
- Genetics
- Radiation Biology
- Toxicology
Background:
- The transgenic Muta mouse is a valuable tool for studying in vivo mutations.
- Understanding radiation-induced mutations is crucial for risk assessment and medical applications.
Purpose of the Study:
- To assess the utility of the Muta mouse for investigating radiation-induced mutations.
- To compare the mutagenic effects of X-rays and ultraviolet (UV) light in vivo and in vitro.
Main Methods:
- Whole-body X irradiation and UVB exposure were applied to Muta mice.
- Mutation frequencies were measured in spleen, liver, and skin tissues.
- UV and X-ray sensitivity was also assessed in cultured embryonic cells.
Main Results:
- Spontaneous mutation frequencies were approximately 7 x 10(-5) in young adults.
- A lethal dose of X-rays (8 Gy) increased mutation frequencies 2.6-3.2 fold in tissues.
- UVB irradiation (10 kJ m-2) increased skin mutation frequency about 6-fold.
- Cultured cells showed a 15-fold increase with UVC (5 J m-2) versus a 2.6-fold increase with 10 Gy X-rays.
Conclusions:
- The Muta mouse is a useful model for studying radiation-induced mutations.
- UV radiation is a more potent mutagen than X-rays in the Muta mouse model.
- The Muta mouse exhibits differential sensitivity to various radiation types.