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Molecular cloning and sequence analysis of the dnaA gene of Staphylococcus aureus
H Katayama1, T Mizushima, T Miki
1Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Biological & Pharmaceutical Bulletin
|July 1, 1997
Abstract:
The dnaA gene of Staphylococcus aureus has now been cloned and sequenced. The deduced amino acid sequence of Staphylococcus aureus DnaA protein is 62% and 39% identical to those of the DnaA protein from Bacillus subtilis and Escherichia coli, respectively. Expression of the dnaA gene of Staphylococcus aureus in Escherichia coli caused lethality in an oriC-dependent manner.