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Related Experiment Videos

Polymorphonuclear leukocyte cells and elastase in tears

M Sakata1, R A Sack, S Sathe

  • 1State University of New York, College of Optometry, Department of Biological Sciences, NY 10010, USA.

Current Eye Research
|August 1, 1997
PubMed
Summary
This summary is machine-generated.

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Overnight eye closure increases caseinolytic activity in tears, primarily from polymorphonuclear leukocyte (PMN) elastase. Sampling methods significantly impact the detection of tear fluid enzymes.

Area of Science:

  • Ophthalmology
  • Biochemistry
  • Cell Biology

Background:

  • Tear fluid contains enzymes that can degrade proteins, influencing ocular surface health.
  • Understanding enzyme activity in tears is crucial for diagnosing and managing eye conditions.

Purpose of the Study:

  • To investigate how different tear collection methods and overnight eye closure affect caseinolytic activity.
  • To identify the specific enzymes responsible for caseinolysis in tear fluid.

Main Methods:

  • Tears were collected using microcapillary tubes or Schirmer strips under reflex, open, and closed eye conditions.
  • Collected samples were analyzed using zymography, SDS-PAGE, and immunoblotting to detect enzyme activity and presence.
  • Immunoprecipitation was used to isolate and identify specific enzymes like plasmin and elastase.

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Main Results:

  • Closed eye samples showed increased plasminogen and plasmin, along with significant caseinolytic activity.
  • The primary source of caseinolytic activity in closed eye samples was identified as polymorphonuclear leukocyte (PMN) elastase.
  • Elastase was also found on Schirmer strips, originating from intact PMNs adhering during collection.

Conclusions:

  • Overnight eye closure promotes the recruitment, activation, and degranulation of PMNs, leading to increased elastase in tear fluid.
  • Elastase is the main contributor to caseinolytic activity in closed eye tear samples.
  • The presence of elastase on Schirmer strips suggests low-level PMN recruitment in open eye conditions.