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Construction of Escherichia coli-Bifidobacterium longum shuttle vector transforming B. longum 105-A and 108-A
H Matsumura1, A Takeuchi, Y Kano
1Department of Molecular Genetics, Kyoto Pharmaceutical University, Japan.
Bioscience, Biotechnology, and Biochemistry
|July 1, 1997
Abstract:
A shuttle vector, pBLES100, was constructed by cloning a Bifidobacterium longum plasmid and a gene encoding spectinomycin adenyltransferase AAD(9) from Enterococcus faecalis into the Escherichia coli vector pBR322. Stable transformants with this plasmid were obtained with an efficiency of 2.2 x 10(4) transformants/microgram DNA or 6.9 x 10(-5) transformants/cell/microgram DNA under the optimal conditions of 10.0 kV/cm, 200 omega, and 25 microF, using B. longum 105-A harvested at late log phase of growth.