Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Complement bridges between cells analysis of a possible cell-cell interaction mechanism

M P Dierich, B Landen

    The Journal of Experimental Medicine
    |December 1, 1977
    PubMed
    Summary

    Complement component C3 facilitates leukocyte rosette formation with complement-coated erythrocytes. This C3-mediated cell binding is temperature and time-dependent, suggesting a role in cell interactions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Absence of indigenous specific West Nile virus antibodies in Tyrolean blood donors.

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2011
    Same author

    A 1-year Aspergillus terreus surveillance study at the University Hospital of Innsbruck: molecular typing of environmental and clinical isolates.

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2008
    Same author

    Ointments as a source of meticillin-resistant Staphylococcus aureus spread.

    The Journal of hospital infection·2008
    Same author

    Immune evasion by acquisition of complement inhibitors: the mould Aspergillus binds both factor H and C4b binding protein.

    Molecular immunology·2007
    Same author

    Influence of amphotericin B and amphotericin B colloidal dispersion on the functions of human phagocytes in defence against Aspergillus species.

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2007
    Same author

    Targeting human immunodeficiency virus type 1 with antibodies derived from patients with connective tissue disease.

    Lupus·2007

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Leukocyte interactions are crucial for immune responses.
    • Complement system proteins play diverse roles in immunity and cell signaling.

    Purpose of the Study:

    • To investigate the role of complement component C3 in leukocyte-erythrocyte rosette formation.
    • To explore the mechanism of C3-mediated cell-cell adhesion.

    Main Methods:

    • Incubation of various leukocytes with C3.
    • Formation of rosettes with complement-coated erythrocytes (EAC142).
    • Assessment of C3 loading, temperature, and time dependency.

    Main Results:

    • Leukocytes coated with C3 formed rosettes with EAC142.
    • Rosette formation percentage correlated with C3 amount.
    • C3 loading was time- and temperature-dependent.
    • C3b did not mediate rosette formation despite receptor binding.

    Conclusions:

    • Complement component C3 mediates leukocyte-erythrocyte rosette formation.
    • Cell-cell adhesion may involve enzymes liberating binding groups on complement components.
    • This mechanism could be relevant for in vivo cell interactions.

    Related Experiment Videos