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Size-selective derivatizations with polymer immobilized reagents

M Szulc1, P Swett, I S Krull

  • 1Department of Chemistry, Northeastern University, Boston, MA 02115, USA.

Biomedical Chromatography : BMC
|July 1, 1997
PubMed
Summary

Immobilized reagents on polymer supports enable size-selective derivatization. This method controls analyte access, allowing selective reactions even with bulky interferents like adamantanamine.

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Area of Science:

  • Analytical Chemistry
  • Polymer Science
  • Organic Chemistry

Background:

  • Traditional derivatization methods can lack selectivity, especially in complex mixtures.
  • Macroporous polymer supports offer a platform for immobilizing reagents with controlled accessibility.

Purpose of the Study:

  • To develop and characterize size-selective derivatization reagents immobilized on macroporous polymer supports.
  • To demonstrate the utility of these reagents in selective analyte derivatization in the presence of sterically hindering compounds.

Main Methods:

  • Immobilization of derivatization reagents onto a macroporous polymer support.
  • Characterization of reaction zones and analyte access based on steric bulk.
  • Application in the derivatization of amines and proteins in the presence of adamantanamine.

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  • Pore size measurements and molecular dimension analysis.

    • Main Results:

    • The immobilized reagents exhibited two distinct reaction zones, enabling size selectivity.
    • Selective derivatization of amines was achieved in the presence of high concentrations of adamantanamine.
    • Analysis of proteins showed reduced derivatization products, indicating reaction at sterically accessible sites only.

    Conclusions:

    • Immobilized reagents on macroporous supports provide a robust platform for size-selective derivatization.
    • This approach enhances selectivity in complex samples by controlling analyte access to reactive sites.
    • The method is applicable to both small molecules and large biomolecules like proteins.