Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Do intact viral particles survive excimer laser ablation?

M J Taravella1, A Weinberg, P Blackburn

  • 1Department of Ophthalmology, University of Colorado Health Sciences Center, Denver, USA.

Archives of Ophthalmology (Chicago, Ill. : 1960)
|August 1, 1997
PubMed
Summary

Excimer laser ablation of varicella-zoster virus did not show evidence of live virus survival, though viral DNA was detected in the laser plume. Safety precautions are still recommended for medical personnel.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Pediatric pelvic trauma].

Der Unfallchirurg·2020
Same author

Organizational uptake of NICE guidance in promoting employees' psychological health.

Occupational medicine (Oxford, England)·2018
Same author

Transcriptome analysis of Porphyromonas gingivalis and Acinetobacter baumannii in polymicrobial communities.

Molecular oral microbiology·2018
Same author

Innate immunity in HIV-1 infection: epithelial and non-specific host factors of mucosal immunity- a workshop report.

Oral diseases·2016
Same author

Transforming growth factor-β1 sustains the survival of Foxp3(+) regulatory cells during late phase of oropharyngeal candidiasis infection.

Mucosal immunology·2015
Same author

Association of Toll-like receptor polymorphisms with HIV status in North Americans.

Genes and immunity·2014

Area of Science:

  • Virology
  • Laser Medicine
  • Infectious Disease Control

Background:

  • Excimer lasers are used in medical procedures.
  • Concerns exist regarding the potential for infectious aerosols generated during laser ablation.
  • Varicella-zoster virus (VZV) is a common human pathogen.

Purpose of the Study:

  • To investigate the survival of infectious varicella-zoster virus after excimer laser ablation.
  • To assess the potential health hazard posed by laser plume to medical personnel.

Main Methods:

  • Fibroblasts infected with attenuated VZV underwent excimer laser ablation.
  • Laser plume was collected and analyzed using polymerase chain reaction (PCR) and viral culture.

Main Results:

Related Experiment Videos

  • Viral DNA fragments were detected in the collected laser plume material.
  • No evidence of infectious, live varicella-zoster virus was found after ablation.

Conclusions:

  • Attenuated varicella-zoster virus appears to be inactivated by excimer laser ablation.
  • Despite findings, use of personal protective equipment like masks is advised during procedures.
  • Further studies are needed to evaluate the effects of excimer laser ablation on other viral agents.