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Updated: Aug 14, 2026
Isolation of Brain-infiltrating Leukocytes
Published on: June 13, 2011
Identification of interleukin-6 producing fibroblastoid cells in cerebrospinal fluid from patients with leukemic
1Second Department of Internal Medicine, Faculty of Medicine, Kagashima University, Japan.
Abstract:
Cytokine producing native cells in cerebrospinal fluid (CSF) have not been identified. So, we investigated the cytokine producing ability of floating cells in CSF from patients with leukemic meningitis. Morphologic study revealed that established cell lines were polygonal or elongated in shape and had an abundant and irregular branched cytoplasm. Immunocytochemical analysis demonstrated positive reactivity with monoclonal anti-fibroblast antibody only. Interleukin-6 (IL-6) was constitutively produced in vitro by these cell lines; both interleukin-1 and lipopolysaccharides significantly increased its synthesis. These findings imply that these fibroblastoid cells are floating in CSF of patients with leukemic meningitis and produce IL-6 in response to various inflammatory stimulations in vivo.
Insights
Researchers identified fibroblastoid cells in cerebrospinal fluid (CSF) of patients with leukemic meningitis. These cells constitutively produce interleukin-6 (IL-6), a key inflammatory cytokine, in response to stimuli.
Area of Science:
- Neuroimmunology
- Cell Biology
- Oncology
Background:
- Native cytokine-producing cells in cerebrospinal fluid (CSF) remain unidentified.
- Leukemic meningitis involves neoplastic infiltration of the central nervous system.
Purpose of the Study:
- To identify and characterize cytokine-producing cells in the CSF of patients with leukemic meningitis.
- To investigate the in vitro production of cytokines by these identified cells.
Main Methods:
- Morphologic and immunocytochemical analysis of established cell lines from patient CSF.
- In vitro culture of CSF cells to assess cytokine production.
- Stimulation assays using interleukin-1 and lipopolysaccharides.
Main Results:
- Fibroblastoid cells were identified in CSF, exhibiting polygonal or elongated shapes with abundant cytoplasm.
- Immunocytochemistry confirmed these cells reacted positively with anti-fibroblast antibodies.
- These fibroblastoid cells constitutively produced interleukin-6 (IL-6) in vitro.
- Interleukin-1 and lipopolysaccharides significantly enhanced IL-6 synthesis.
Conclusions:
- Fibroblastoid cells are present in the CSF of patients with leukemic meningitis.
- These cells possess the capacity to produce IL-6, a crucial inflammatory mediator.
- CSF IL-6 production by these cells may be modulated by inflammatory signals in vivo.
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