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Search for specificity in natural cell-mediated cytotoxicity
Journal of the National Cancer Institute
|December 1, 1979
Summary
Viral infection does not solely explain antigenicity changes in osteosarcoma cell lines. Natural cell-mediated cytotoxicity (NCMC) may influence these antigen differences, even in uninfected cells.
Area of Science:
- Immunology
- Cell Biology
- Virology
Background:
- Osteosarcoma cell lines exhibit distinct antigenicity.
- Feline sarcoma virus infection can alter cell surface properties.
Purpose of the Study:
- To investigate antigenicity differences between human osteosarcoma cells and virus-infected sublines.
- To determine if viral infection is the sole cause of observed antigenicity changes.
Main Methods:
- Competitive inhibition of natural cell-mediated cytotoxicity (NCMC).
- Absorption and elution of antibodies to identify NCMC specificity.
- Testing antibody-treated cells against TE 85/B and NIH E1041 sublines.
Main Results:
- Detected antigenicity differences between TE 85/B and NIH E1041 cells.
- Observed antigenicity variations among all sublines, including uninfected ones.
- Found that separately cultured sublines became antigenically distinct over time.
Conclusions:
- Viral infection is not necessarily the cause of all observed antigenicity differences.
- Natural cell-mediated cytotoxicity (NCMC) may play a role in controlling cell surface antigens.
- Continuous passage and separate culturing can lead to antigenicity changes independent of viral status.