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The Multifaceted Benefits of Protein Co-expression in Escherichia coli
Published on: February 5, 2015
Cloning and overexpression of polypeptide release factor 1 of Thermus thermophilus
1Department of Tumor Biology, Institute of Medical Science, University of Tokyo, Japan.
Abstract:
A prfA gene encoding polypeptide release factor RF1 was cloned from Thermus thermophilus. T thermophilus RF1 shares 68% homology with Escherichia coli RF1, and its overproduction reduced readthrough translation of UAG, not of UGA, in the lacZ gene. Rapid purification of T thermophilus RF1 was achieved by T7-RNA polymerase driven overexpression of T thermophilus RF1 protein with a C-terminal histidine tag.
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