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Practical method for the multigram separation of the 5- and 6-isomers of carboxyfluorescein
1Medical Biotechnology Center, School of Medicine, University of Maryland, Baltimore 21201, USA. frossi@scuacc.scu.edu
Bioconjugate Chemistry
|July 1, 1997
Abstract:
An efficient preparative method for separating 5- and 6-carboxyfluorescein is presented. 6-Carboxyfluorescein dipivalate is isolated as its diisopropylamine salt, which can be converted to the free acid or used directly in coupling reactions. The 5-isomer is isolated from the acidified mother liquor. Isomerically pure carboxyfluoresceins are prepared by hydrolysis of the corresponding dipivalates.