Multiple sulfa compounds: high-pressure liquid chromatographic assay and mobile phase correlation

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis

Background:

• Sulfonamides are crucial antimicrobial agents.
• Accurate quantification of sulfa drug mixtures is essential for quality control.

Purpose of the Study:

• To develop a rapid high-pressure liquid chromatography (HPLC) assay for a mixture of sulfacetamide, sulfathiazole, and sulfabenzamide.
• To explore the relationship between solvent molar polarization and chromatographic retention times.

Main Methods:

• High-pressure liquid chromatography (HPLC) was employed.
• Solvent molar polarization parameters were systematically varied.
• Retention times of the sulfa drugs were measured.

Main Results:

• A rapid and effective HPLC assay for the target sulfa drug mixture was successfully developed.
• A significant linear correlation was established between solvent molar polarization and drug retention times.
• This correlation allows for predictable adjustments to chromatographic conditions.

Conclusions:

• The developed HPLC method offers a fast and reliable approach for analyzing the specified sulfa drug combination.
• The observed linear relationship between molar polarization and retention time provides a valuable predictive tool for chromatographic method development.
• This predictive capability can streamline the optimization of analytical methods for similar compounds.