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Related Experiment Videos

A pilot study identifying type V collagenolytic activity in human amniotic fluid

W J Polzin1, E G Lockrow, W K Morishige

  • 1Department of Obstetrics and Gynecology, Tripler Army Medical Center, Tripler AMC, Hawaii, USA.

American Journal of Perinatology
|February 1, 1997
PubMed
Summary

This study investigated type V collagenase activity in amniotic fluid, which may be linked to preterm premature rupture of membranes. Finding this activity could explain how the amniotic sac breaks down during pregnancy complications.

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Area of Science:

  • Obstetrics and Gynecology
  • Biochemistry
  • Extracellular Matrix Biology

Background:

  • Amniorrhexis, or rupture of membranes, complicates pregnancies, especially preterm ones.
  • The amniotic extracellular matrix comprises collagen types I-V.
  • Collagenases for types I-IV collagen are present in amniotic fluid, but type V collagenase activity is unknown.

Purpose of the Study:

  • To investigate the presence and activity of type V collagenase in human amniotic fluid.
  • To determine if type V collagenolytic activity is associated with different pregnancy states.

Main Methods:

  • Collected human amniotic fluid from pregnancies in various clinical conditions.
  • Assayed amniotic fluid for type V collagenolytic activity using specific biochemical methods.

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Main Results:

  • Evidence of type V collagenolytic activity was detected in human amniotic fluid.
  • The activity varied depending on the clinical state of the pregnancy.

Conclusions:

  • Type V collagen plays a crucial role in maintaining amniotic sac integrity.
  • The presence of type V collagenase in amniotic fluid may contribute to amniorrhexis and pregnancy complications.