Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD6 recognizes the neural adhesion molecule BEN

J E Skonier1, M A Bowen, A Aruffo

  • 1Bristol-Myers Squibb Pharmaceutical Research Institute (BMS-PRI), Seattle, Washington 98121, USA.

Protein Science : a Publication of the Protein Society
|August 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Activity Landscapes, Information Theory, and Structure - Activity Relationships.

Molecular informatics·2016
Same author

Substrate specificity of human matriptase-2.

Biochimie·2013
Same author

Use of monoclonal antibodies for expression cloning.

Current protocols in immunology·2008
Same author

Transient expression of proteins using COS cells.

Current protocols in neuroscience·2008
Same author

Computational techniques for diversity analysis and compound classification.

Mini reviews in medicinal chemistry·2004
Same author

Mini-fingerprints for virtual screening: design principles and generation of novel prototypes based on information theory.

SAR and QSAR in environmental research·2003
Same journal

Macromolecular crowding inhibits degradation of alpha-synuclein amyloid fibrils induced by cathepsins and MMP9.

Protein science : a publication of the Protein Society·2026
Same journal

Sequence-encoded differences in the conformational ensembles of CITED transcriptional activation domains impact coactivator binding.

Protein science : a publication of the Protein Society·2026
Same journal

The phospholipid biosynthesis enzyme PlsB contains three distinct domains for membrane association, lysophosphatidic acid synthesis, and dimerization.

Protein science : a publication of the Protein Society·2026
Same journal

Structural basis of ligand selectivity in FAD/NAD(P)H-dependent dehydrogenases: insights from trypanothione reductase and type II NADH dehydrogenase.

Protein science : a publication of the Protein Society·2026
Same journal

Achieving protease substrate-specific inhibition by mAb dual functional selections.

Protein science : a publication of the Protein Society·2026
Same journal

How important are quantum mechanical effects in controlling biological functions: Enzymes, electron transfer and bird navigation.

Protein science : a publication of the Protein Society·2026
See all related articles

The immune cell receptor CD6 binds to both ALCAM and the evolutionarily conserved chicken protein BEN. This cross-species binding suggests a fundamental role for CD6-ligand interactions in biology.

Area of Science:

  • Immunology
  • Neuroscience
  • Cell Adhesion

Background:

  • CD6 and its ligand, activated leukocyte cell adhesion molecule (ALCAM, CD166), are present on immune cells and in the brain.
  • CD6-ligand interactions are known to regulate T cell function.
  • ALCAM shares structural similarities with the chicken neural adhesion molecule BEN.

Purpose of the Study:

  • To investigate the binding interaction between CD6 and BEN.
  • To compare the binding avidity of CD6 to ALCAM and BEN.
  • To explore the evolutionary conservation of CD6-ligand interactions.

Main Methods:

  • Utilized fusion proteins containing full-length extracellular regions of ALCAM and BEN.
  • Assessed binding avidity of CD6 to ALCAM and BEN.

Related Experiment Videos

  • Analyzed sequence homology and domain organization.

    • Main Results:

    • CD6 specifically binds to BEN, though with lower avidity than ALCAM.
    • No significant differences in binding avidity were observed between ALCAM and BEN fusion proteins with full-length extracellular regions.
    • Homotypic interactions between full-length forms likely influence binding avidity.

    Conclusions:

    • CD6-ligand interactions are conserved across species, as evidenced by the CD6-BEN interaction.
    • The findings suggest a conserved biological function for CD6-ligand pathways.
    • Homotypic interactions play a crucial role in modulating binding avidity.