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Related Experiment Videos

Catfish thrombocytes express an integrin-like CD41/CD61 complex

B J Passer1, C H Chen, N W Miller

  • 1Department of Microbiology, University of Alabama at Birmingham, 35294, USA.

Experimental Cell Research
|August 1, 1997
PubMed
Summary
This summary is machine-generated.

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Researchers identified a specific antigen on catfish thrombocytes using monoclonal antibodies. This antigen, homologous to the human CD41/CD61 complex, plays a role in thrombocyte aggregation and shape change.

Area of Science:

  • Immunology
  • Comparative Biology
  • Molecular Biology

Background:

  • Thrombocytes (platelets) are crucial for hemostasis in vertebrates.
  • The CD41/CD61 complex, composed of alphaIIb and beta3 integrin subunits, is a key platelet surface glycoprotein in mammals.
  • Understanding the conservation of this complex in non-mammalian vertebrates provides insights into its evolutionary history.

Purpose of the Study:

  • To identify and characterize a thrombocyte-specific antigen in catfish (Ictalurus punctatus and Ictalurus furcatus).
  • To investigate the molecular composition and functional properties of this antigen.
  • To determine if this antigen represents a conserved homolog of the mammalian CD41/CD61 complex.

Main Methods:

  • Monoclonal antibodies (4-20 and 7-2) were used to immunoprecipitate the target antigen from catfish thrombocytes.

Related Experiment Videos

  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing and non-reducing conditions was employed to analyze the glycoprotein chains.
  • N-terminal amino acid sequencing was performed to identify homologous protein subunits.
  • Functional assays were conducted to assess the antibodies' effect on thrombocyte aggregation and cell shape.

    • Main Results:

    • A thrombocyte-specific antigen was identified in two catfish species.
    • The antigen consists of two noncovalently associated glycoprotein chains (Mr 180,000 and Mr 95,000).
    • Under reducing conditions, the Mr 180,000 chain dissociates into Mr 150,000 and 32,000 subcomponents.
    • N-terminal sequencing revealed homology of the Mr 95,000 chain with beta3 integrin and the Mr 150,000 chain with alphaIIb integrin.
    • The specific antibodies induced catfish thrombocyte aggregation and altered cell shape.

    Conclusions:

    • The identified catfish thrombocyte antigen is homologous to the alphaIIb/beta3 integrin complex, also known as the CD41/CD61 complex in mammals.
    • This study provides evidence for the evolutionary conservation of the CD41/CD61 complex in bony fish.
    • The findings highlight the conserved role of this complex in thrombocyte function across diverse vertebrate species.