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Cryo-electron microscopy structure of yeast Ty retrotransposon virus-like particles
K J Palmer1, W Tichelaar, N Myers
1Department of Crystallography, Birkbeck College, London, United Kingdom.
Journal of Virology
|September 1, 1997
Abstract:
The virus-like particles (VLPs) produced by the yeast retrotransposon Ty1 are functionally related to retroviral cores. These particles are unusual in that they have variable radif. A paired mass-radius analysis of VLPs by scanning transmission electron microscopy showed that many of these particles form an icosahedral T-number series. Three-dimensional reconstruction to 38-A resolution from cryo-electron micrographs of T = 3 and T = 4 shells revealed that the single structural protein encoded by the TYA gene assembles into spiky shells from trimeric units.