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Related Experiment Videos

Gene transfer, gentamicin resistance and enterococci

S Simjee1, M J Gill

  • 1University of Birmingham, Medical School, Department of Infection, Edgbaston, England.

The Journal of Hospital Infection
|August 1, 1997
PubMed
Summary

Enterococci are difficult-to-treat pathogens that acquire antibiotic resistance. The AAC6'-APH2" enzyme rapidly spreads, conferring high-level gentamicin resistance and potentially transferring to other bacteria.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Enterococci are adaptable pathogens with intrinsic antibiotic resistance and environmental tolerance.
  • Increasing difficulty in treating enterococcal infections due to acquired antibiotic-resistance genes.
  • Rapid dissemination of the AAC6 -APH2" bifunctional enzyme is a growing concern.

Purpose of the Study:

  • To investigate the spread and implications of the AAC6 -APH2" enzyme in enterococci.

Main Methods:

  • Analysis of antibiotic resistance gene acquisition in enterococci.
  • Tracking the dissemination of the AAC6 -APH2" gene.
  • Investigating the association of the resistance gene with mobile genetic elements like plasmids and transposons.

Main Results:

  • The AAC6 -APH2" enzyme confers high-level resistance to gentamicin and related aminoglycosides (excluding streptomycin).
  • The gene encoding this enzyme is found on both narrow and broad host range plasmids.
  • The resistance gene has recently been identified on conjugative transposons.

Conclusions:

  • The AAC6 -APH2" enzyme's presence on mobile genetic elements facilitates its rapid spread.
  • Conjugative transposons carrying this gene raise concerns about inter-species transfer to other pathogenic bacteria.
  • Understanding the dissemination mechanisms is crucial for combating enterococcal antibiotic resistance.

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