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Related Experiment Videos

Decrease in Thy-1 expression on peripheral CD34 positive cells induced by G-CSF mobilization. The Tohoku Children

S Tsuchiya1, A Kikuta, Y Shimizu

  • 1Department of Pediatric Oncology, Tohoku University, Sendai, Japan. tsuchiya@europa.idac.tohoku.ac.jp

The Tohoku Journal of Experimental Medicine
|June 1, 1997
PubMed
Summary

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Mobilizing hematopoietic progenitor cells involves changes in surface markers. A decrease in Thy-1 (CDw90) expression on CD34+ cells correlates with increased cell mobilization after G-CSF treatment.

Area of Science:

  • Hematology
  • Immunology
  • Cell Biology

Background:

  • Peripheral hematopoietic progenitor cells (PHPC) are crucial for reconstituting the immune system after chemotherapy.
  • Mobilization of PHPC using granulocyte-colony stimulating factor (G-CSF) and chemotherapy is a standard procedure.
  • Understanding the cytological features of mobilized PHPC is essential for optimizing treatment strategies.

Purpose of the Study:

  • To investigate the expression of lineage- and progenitor cell-specific surface markers on CD34+ cells mobilized by chemotherapy and G-CSF.
  • To identify changes in surface marker expression that may be associated with the mobilization process.

Main Methods:

  • Peripheral blood samples were collected from patients undergoing chemotherapy and G-CSF treatment for various cancers.

Related Experiment Videos

  • Flow cytometry was used to analyze the expression of surface markers, including CD34, CD38, HLA-DR, CD19, CD117, and Thy-1 (CDw90), on PHPC.
  • Samples were analyzed at different time points to assess changes during mobilization.

    • Main Results:

    • CD34+ PHPC cells consistently expressed CD38 (>95%) and HLA-DR (~85%).
    • CD19 was expressed on the majority of CD34+ cells, while CD117 was found on 10-20%.
    • A significant decrease in the Thy-1 (CDw90)+/CD34+ cell population was observed at the peak of mobilization compared to the early phase after G-CSF administration (20% vs. 60%).

    Conclusions:

    • The expression profile of CD34+ PHPC cells includes CD38, HLA-DR, CD19, and CD117.
    • The observed decrease in Thy-1 (CDw90) expression on CD34+ cells during peak mobilization suggests a role in facilitating their release into peripheral blood.
    • These findings contribute to understanding the mechanisms of G-CSF-induced stem cell mobilization.