Cell-mediated immune responses to liver membrane protein in canine chronic hepatitis

Peripheral blood mononuclear cells from dogs with chronic inflammatory liver disease and dogs with noninflammatory liver diseases were evaluated for proliferative responses to pokeweed mitogen and canine liver membrane protein. Dogs with chronic hepatitis were selected based on histopathological evidence of periportal lymphocytic infiltrates with or without neutrophilic infiltrates, fibrosis and necrosis. Incorporation of tritiated thymidine was assessed 72 hours after addition of liver membrane protein or pokeweed mitogen. Peripheral blood mononuclear cell proliferation in response to liver membrane protein was significantly higher in chronic hepatitis dogs compared to control dogs. Eight of 12 dogs with chronic hepatitis and 2 of 7 dogs with noninflammatory liver disease had proliferative responses to liver membrane protein greater than 2 standard deviations above the mean of the control group. These data support the hypothesis that immune-mediated processes are involved in canine chronic hepatitis, but did not determine whether this is a primary disorder or occurred secondary to liver destruction.