Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tracing the Na/K-ATPase with rubidium

F X Beck1, K Thurau

  • 1Department of Physiology, University of Munich, Federal Republic of Germany.

Wiener Klinische Wochenschrift
|June 27, 1997
PubMed
Summary

Rubidium uptake in kidney cells reveals that intracellular sodium concentration regulates the Na/K(Rb)-ATPase pump. Chronic sodium changes trigger adaptations for sustained sodium transport.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Immunohistochemical analysis on potential new molecular targets for esophageal cancer.

Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus·2013
Same author

[Patient information discussion].

Klinische Monatsblatter fur Augenheilkunde·2011
Same author

Ammonium affects tight junctions and the cytoskeleton in MDCK cells.

Pflugers Archiv : European journal of physiology·2005
Same author

Recovery of cell volume and electrolytes of A6 cells after re-establishing isotonicity following hypotonic stress.

Pflugers Archiv : European journal of physiology·2003
Same author

Heat shock proteins and the cellular response to osmotic stress.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology·2000
Same author

Blood pressure and indices of glomerular filtration area in hypertensive and normotensive Prague rats.

Kidney & blood pressure research·2000

Area of Science:

  • Nephrology
  • Cell Physiology
  • Biochemistry

Background:

  • The Na/K-ATPase is crucial for maintaining cellular ion balance in renal tubule cells.
  • Understanding its regulation by intracellular sodium is key to renal function.

Purpose of the Study:

  • To investigate the role of intracellular sodium concentration in regulating Na/K(Rb)-ATPase activity.
  • To assess adaptive mechanisms in renal tubules under altered sodium conditions.

Main Methods:

  • Utilized rubidium (Rb) as a potassium (K) marker ion.
  • Measured initial Rb uptake rates in single rat kidney tubule cells using electron microprobe analysis.
  • Examined conditions of acute stimulation and inhibition of transepithelial sodium (Na) absorption.

Main Results:

  • Rubidium uptake rates strongly correlated with intracellular Na concentrations.
  • Intracellular Na concentration was identified as a primary determinant of Na/K(Rb)-ATPase activity.
  • Chronic increased distal Na delivery induced adaptive processes maintaining Na transport at normal intracellular Na levels.

Conclusions:

  • Na/K(Rb)-ATPase activity is precisely adjusted by intracellular Na concentration to balance Na entry and extrusion.
  • Renal tubules adapt to chronic sodium changes, enabling sustained transcellular Na movement.

Related Experiment Videos