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Immunohistochemical processing of tissue which allows for embedding in Araldite, and orientation and correlation
1Department of Physiology, University of Western Australia, Nedlands, Australia. poates@uniwa.uwa.edu.au
Journal of Microscopy
|July 1, 1997
Abstract:
This paper describes a simple and efficient method for the immunohistochemical processing of tissue sections on glass slides and the subsequent transfer of the entire section to an Araldite medium. These sections present in one plane can then be orientated and when cut provide the user with whole tissue, free from gaps due to folding. This procedure therefore enables the changes in protein concentration and distribution to be assessed and correlated within and between different cells at the light and electron microscopic level. This procedure may be of use in other microscopic techniques studies.