[Fast DNA amplification in small ultrathin-wall microplates]

A new method was developed for fast DNA amplification by polymerase chain reaction in tiny ultrathin microplates formed directly on the thermocycler's thermoblock. The microplates are made from thin (40-60 microns) polypropylene film by the thermal vacuum-formation method. Due to the effective heat transfer to 10-15 microliters samples and a high velocity of heating and cooling of the thermoblock (up to 7 degrees C/s), the total duration of the DNA amplification (30 cycles) is only 15-30 min.