Related Experiment Videos
Reliable determination of binding affinity and kinetics using surface plasmon resonance biosensors
1Biomedical Engineering and Instrumentation Program, National Institutes of Health, Bethesda, MD 20892-5766, USA. pschuck@helix.nih.gov
Current Opinion in Biotechnology
|August 1, 1997
Abstract:
Progress has been made in the identification of experimental and analytical procedures that allow for a more reliable determination of equilibrium and kinetic constants. Possible origins of the frequently observed deviations of the measured binding progress from that expected for chemical binding of pseudo-first order, and appropriate experimental controls have been proposed. Improved analytical approaches include the application of global analysis and analytical corrections for the influence of mass transport.