Polyploid-specific repetitive DNA sequences from triploid ginbuna (Japanese silver crucian carp, Carassius auratus...

Repetitive DNA sequences (Cal3nDr) in the genome of a triploid ginbuna (Carassius auratus langsdorfi) were isolated from the DraI digests of the genomic DNA. This AT-rich (61%) Cal3nDr monomer was 137 bp in length. The nucleotide similarity among the monomers from the same individual was considerably high (above 97%). Hybridization analyses revealed that the Cal3nDr sequences were organized into tandem arrays. These DNA sequences were present only in triploid and tetraploid ginbunas and were absent from diploid ginbuna, gengorobuna, goldfish, and other cyprinid fishes, and therefore appeared to be specific to polyploid ginbunas. In situ hybridization data showed their localization on one to four out of a total of 150 to 156 chromosomes, depending on the individuals or clonal lines, of the triploid ginbuna. The origin of the Cal3nDr sequences is also discussed on the basis of observation of the artificial triploid ginbuna produced by crossing a diploid female with a tetraploid male.