Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

3D structural information as a guide to protein engineering using genetic selection

P Kast1, D Hilvert

  • 1Department of Molecular Biology, Scripps Research Institute, La Jolla, CA 92037, USA. pjokast@scripps.edu

Current Opinion in Structural Biology
|August 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protein Nanocontainers from Nonviral Origin: Testing the Mechanics of Artificial and Natural Protein Cages by AFM.

The journal of physical chemistry. B·2016
Same author

Variations in truncal body circumferences affect fat mass quantification with bioimpedance analysis.

European journal of clinical nutrition·2011
Same author

Shape complementarity, binding-site dynamics, and transition state stabilization: a theoretical study of Diels-Alder catalysis by antibody 1E9.

Chembiochem : a European journal of chemical biology·2002
Same author

Improved synthesis of C-terminal peptide thioesters on "safety-catch" resins using LiBr/THF.

Organic letters·2001
Same author

Searching sequence space for protein catalysts.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

Catalysis of decarboxylation by a preorganized heterogeneous microenvironment: crystal structures of abzyme 21D8.

Journal of molecular biology·2001
Same journal

Tomogram exploration through template matching and deep learning.

Current opinion in structural biology·2026
Same journal

A comparative review of cryo-electron ptychography: Biological applications and future perspectives.

Current opinion in structural biology·2026
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
Same journal

Tuning the physicochemical properties of rationally designed protein-based biomolecular condensates.

Current opinion in structural biology·2026
See all related articles

Structure-guided evolution uses 3D protein information to guide mutagenesis and select active variants. This protein engineering approach aids in understanding protein function and design.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Engineering

Background:

  • Protein systems are crucial for biological functions.
  • Understanding protein structure-mechanistic relationships is vital for biological research and biotechnology.

Purpose of the Study:

  • To investigate protein systems using structure-guided evolutionary strategies.
  • To characterize structural and mechanistic features of proteins.
  • To explore implications for protein design and engineering.

Main Methods:

  • Utilizing available 3D structural information of proteins.
  • Targeting critical protein regions for randomizing mutagenesis.
  • Selecting for active variants of corresponding genes.

Related Experiment Videos

Main Results:

  • A variety of protein systems have been investigated.
  • Structure-guided evolutionary strategies have been successfully applied.
  • Identification of critical regions for protein functional characterization.

Conclusions:

  • Structure-guided evolutionary strategies are effective for protein investigation.
  • These approaches enhance the characterization of protein structural and mechanistic features.
  • Significant implications for the rational design of novel protein functions and systems.