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T-2 toxin-induced DNA damage in mouse livers: the effect of pretreatment with coenzyme Q10 and alpha-tocopherol

F Atroshi1, A Rizzo, I Biese

  • 1Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Helsinki, Finland.

Molecular Aspects of Medicine
|January 1, 1997
PubMed

Insights

T-2 toxin causes significant liver DNA damage and depletes glutathione. Antioxidants coenzyme Q10 (CoQ10) and alpha-tocopherol (vitamin E) offered protection against T-2 toxin-induced hepatic DNA damage and cell death.

Area of Science:

  • Toxicology
  • Hepatology
  • Biochemistry

Background:

  • Active oxygen species are implicated in organ damage.
  • T-2 toxin is a known mycotoxin that can cause cellular injury.

Purpose of the Study:

  • To investigate the role of oxidative stress in T-2 toxin-induced hepatic DNA damage.
  • To evaluate the protective effects of coenzyme Q10 (CoQ10) and alpha-tocopherol (vitamin E) against T-2 toxin-induced liver damage.

Main Methods:

  • Mice were administered single oral doses of T-2 toxin (1.8 or 2.8 mg/kg).
  • Hepatic DNA fragmentation and glutathione (GSH) levels were measured.
  • Mice were pretreated with CoQ10 (6 mg/kg) and alpha-tocopherol (6 mg/kg) before T-2 toxin administration.

Main Results:

  • T-2 toxin induced 76% hepatic DNA fragmentation.
  • T-2 toxin significantly decreased hepatic GSH levels.
  • CoQ10 and alpha-tocopherol pretreatment reduced DNA damage and GSH depletion.

Conclusions:

  • Oxidative damage is a key mechanism in T-2 toxin-induced liver injury and DNA damage.
  • CoQ10 and vitamin E demonstrate protective potential against T-2 toxin toxicity.
  • T-2 toxin-induced hepatic DNA damage may contribute to hepatocarcinogenesis.

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