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Related Experiment Videos

Prolactin receptor expression in the developing mouse embryo

S J Tzeng1, D I Linzer

  • 1Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208, USA.

Molecular Reproduction and Development
|September 1, 1997
PubMed
Summary
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Prolactin receptor expression in mouse embryos shows dynamic changes, with distinct patterns in fetal tissues and the placenta. These findings suggest a crucial role for placental lactogens in fetal development.

Area of Science:

  • Developmental Biology
  • Endocrinology
  • Molecular Biology

Background:

  • Prolactin is a key hormone regulating various physiological processes.
  • The prolactin receptor (PRLR) mediates prolactin signaling.
  • Understanding PRLR developmental expression is crucial for fetal development research.

Purpose of the Study:

  • To investigate the temporal and spatial expression patterns of the prolactin receptor during mouse embryonic development.
  • To identify specific fetal tissues with significant PRLR expression.
  • To explore the implications of PRLR expression for fetal development and the role of placental lactogens.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) for mRNA quantification.
  • In situ hybridization for spatial localization of mRNA.

Related Experiment Videos

  • Radioligand binding assays for receptor protein detection.

    • Main Results:

    • Prolactin receptor mRNA levels in embryos exhibited a dip around embryonic day 14, contrasting with constant placental levels.
    • A switch in PRLR isoform dominance occurred in the fetal liver from long to short forms between embryonic days 16 and 18.
    • Widespread PRLR mRNA and protein expression was observed in fetal tissues, notably in bone, cartilage, thymus, pituitary, tongue, skeletal muscle, and brain regions.

    Conclusions:

    • The developmental expression profile of prolactin receptors in mouse embryos is complex and temporally regulated.
    • Specific PRLR isoforms show dynamic changes in abundance in fetal tissues like the liver.
    • The expression pattern supports a significant role for placental lactogens in mediating prolactin's effects on fetal growth and development.