Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Beta amyloid toxicity does not require RAGE protein

Y Liu1, R Dargusch, D Schubert

  • 1The Salk Institute for Biological Studies, La Jolla California 92037, USA.

Biochemical and Biophysical Research Communications
|August 8, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Yield of dwarf tomatoes grown with a nutrient solution based on recycled synthetic urine.

Life sciences in space research·2019
Same author

Scaling of the surface vasculature on the human placenta.

Physical review. E·2018
Same author

Parvalbumin- and vasoactive intestinal polypeptide-expressing neocortical interneurons impose differential inhibition on Martinotti cells.

Nature communications·2016
Same author

Review and analysis of over 40 years of space plant growth systems.

Life sciences in space research·2016
Same author

Cadherin-13, a risk gene for ADHD and comorbid disorders, impacts GABAergic function in hippocampus and cognition.

Translational psychiatry·2015
Same author

Activating PI3Kδ mutations in a cohort of 669 patients with primary immunodeficiency.

Clinical and experimental immunology·2015

Receptor for advanced glycation end products (RAGE) is not the neural receptor for amyloid beta (Aβ) protein. Studies found no RAGE mRNA in neural cells, and Aβ toxicity was unaffected by RAGE inhibition.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Amyloid beta (Aβ) protein is implicated in neurodegenerative diseases.
  • Receptor for advanced glycation end products (RAGE) has been proposed as a neural receptor for Aβ.

Purpose of the Study:

  • To investigate whether RAGE is the neural receptor for Aβ.

Main Methods:

  • Polymerase chain reaction (PCR) and northern blot analysis to detect RAGE mRNA in neural cell lines and rat cortical neurons.
  • Assessing the effect of trypsin treatment on Aβ toxicity.
  • Evaluating the impact of glycated albumin on the Aβ response.

Main Results:

  • RAGE mRNA was not detected in cultured neural cells, though it was present in lung tissue.

Related Experiment Videos

  • Trypsin treatment, which affects cell surface RAGE, did not alter Aβ toxicity.
  • Glycated albumin, a RAGE ligand, did not modify the Aβ response.

    • Conclusions:

    • RAGE is not the neural receptor responsible for mediating the effects of Aβ.
    • These findings challenge the proposed role of RAGE in Aβ-induced neural toxicity.