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Analysis of relationship between programmed cell death and cell cycle in limb-bud
1Department of Radiation Research, Tokyo Metropolitan Institute of Medical Science, Japan. ebot35@holyrood.ed.ac.uk
Hormone Research
|January 1, 1997
Summary
Programmed cell death and cell cycle are linked in amniote limb development. A critical S-phase was identified as the most sensitive time to inhibit cell death using 5-bromodeoxyuridine (BrdU).
Area of Science:
- Developmental Biology
- Cell Biology
- Molecular Biology
Background:
- Programmed cell death is essential for amniote limb morphogenesis.
- Understanding the interplay between cell cycle and apoptosis is crucial for developmental processes.
Purpose of the Study:
- To investigate the relationship between cell cycle and programmed cell death during limb development.
- To identify critical phases of the cell cycle sensitive to apoptosis inhibition.
Main Methods:
- Utilized microfluorometry to measure 5-bromodeoxyuridine (BrdU) incorporation.
- Quantified DNA content of individual cells to analyze cell cycle distribution.
- Administered excess 5-bromodeoxyuridine (BrdU) to assess its effect on cell death.
Main Results:
- A critical S-phase was identified, representing the most sensitive period for inhibiting cell death with excess 5-bromodeoxyuridine (BrdU).
- Cells committed to apoptosis were observed to exit the cell cycle at the G2-phase.
- Demonstrated a close relationship between cell cycle regulation and programmed cell death in limb-buds.
Conclusions:
- Programmed cell death and cell cycle are intricately linked events during amniote limb morphogenesis.
- Cells undergoing apoptosis are withdrawn from the cell cycle at the G2-phase.
- Nuclear events in apoptosis during limb development are closely related to cell cycle progression.