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Glucose-dependent liver gene expression in upstream stimulatory factor 2 -/- mice
V S Vallet1, A A Henrion, D Bucchini
1Institut Cochin de Génétique Moléculaire, Université René Descartes, 24 rue du Faubourg Saint-Jacques, 75014 Paris, France.
The Journal of Biological Chemistry
|August 29, 1997
Summary
Upstream stimulatory factors (USF) 2 are essential for the liver's transcriptional response to glucose. This study demonstrates that USF2 proteins are required in vivo for regulating key metabolic genes, providing crucial insights into nutrient gene regulation.
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- Upstream stimulatory factors (USF) 1 and 2 are Myc family transcription factors that bind E boxes.
- USF factors activate gene transcription and are implicated in glucose-responsive gene regulation in the liver.
- The in vivo requirement for USF proteins in nutrient gene regulation has not been established.
Purpose of the Study:
- To investigate the in vivo role of USF1 and USF2 in gene expression.
- To determine if USF proteins are necessary for the transcriptional response of genes to glucose in the liver.
Main Methods:
- Generation of USF2 knockout (USF2 -/-) mice using homologous recombination.
- Analysis of the transcriptional response of target genes in USF2 deficient mice.
Main Results:
- USF2 proteins are demonstrated to be required in vivo.
- The study provides the first evidence that USF2 is essential for the normal transcriptional response of L-type pyruvate kinase and Spot 14 genes to glucose in the liver.
Conclusions:
- USF2 plays a critical role in the in vivo regulation of glucose-responsive genes in the liver.
- These findings highlight the importance of USF2 in nutrient sensing and metabolic control.