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Related Experiment Videos

cDNAs derived from primary and small cytoplasmic Alu (scAlu) transcripts

T H Shaikh1, A M Roy, J Kim

  • 1Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, 1901 Perdido St, New Orleans, LA, 70112, USA.

Journal of Molecular Biology
|August 15, 1997
PubMed
Summary
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Researchers found that multiple Alu elements are actively transcribed by RNA polymerase III, with a preference for younger subfamilies. This suggests specific sequence elements and flanking regions influence Alu transcription.

Area of Science:

  • Molecular Biology
  • Genomics
  • Evolutionary Biology

Background:

  • Alu elements are abundant retrotransposons in the human genome.
  • Their transcriptional activity and regulation are not fully understood.

Purpose of the Study:

  • To investigate the transcriptional activity of Alu elements.
  • To identify factors influencing Alu transcription and subfamily bias.

Main Methods:

  • Isolation and sequencing of cDNAs derived from primary Alu transcripts.
  • Sequence alignment with Alu consensus sequences.
  • In vitro transcription studies of a specific Alu element.

Main Results:

  • Identified 26 cDNAs from authentic RNA polymerase III-directed Alu transcripts.

Related Experiment Videos

  • Demonstrated transcriptional activity from multiple Alu loci, with bias towards younger subfamilies.
  • Showed selection for consensus sequences and promoter elements, and identified flanking sequence influence.

    • Conclusions:

    • Multiple Alu loci are transcriptionally active, regulated by specific sequence features.
    • Transcriptional activity is biased towards evolutionarily younger Alu subfamilies.
    • Flanking sequences play a role in the transcriptional activity of Alu elements.