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Interaction of DA41, a DAN-binding protein, with the epidermal growth factor-like protein, S(1-5)

T Ozaki1, K Kondo, Y Nakamura

  • 1Division of Biochemistry, Chiba Cancer Center Research Institute, 666-2, Nitona, Chuoh-ku, Chiba, 260, Japan.

Biochemical and Biophysical Research Communications
|August 18, 1997
PubMed

Insights

Researchers identified a novel protein, DA41, interacting with the tumor suppressor DAN. This interaction involves the epidermal growth factor-like protein S(1-5) and may regulate cell growth.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • A novel protein, DA41, has been identified and shown to associate with the candidate tumor suppressor DAN protein.
  • The interaction partners of DA41 are not fully characterized, limiting understanding of its cellular function.

Purpose of the Study:

  • To identify proteins that interact with DA41.
  • To elucidate the potential role of DA41 in regulating cell growth through its interactions.

Main Methods:

  • Yeast two-hybrid system screening using a truncated DA41 (1-308) as bait.
  • Screening of an adult rat lung cDNA library.
  • Database search to identify the interacting protein.

Main Results:

  • A positive clone, T16, was identified, containing a 1934-nucleotide cDNA encoding a 493-amino acid protein.
  • T16 showed strong sequence similarity to the human epidermal growth factor (EGF)-like protein, S(1-5).
  • The interaction between DA41 and T16 (S(1-5)) was mapped to amino acids 155-232 of DA41.

Conclusions:

  • DA41 interacts with the human epidermal growth factor (EGF)-like protein S(1-5).
  • Given that DAN suppresses and S(1-5) stimulates DNA synthesis, their interaction via DA41 may regulate cell growth.

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