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Updated: Aug 18, 2026

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps
Published on: August 18, 2012
A search for the intermediate radical, ONOO, in the reaction between oxygen and nitric oxide in solution
1Inflammation Research Group, St. Bartholomew's Hospital School of Medicine and Dentistry, London, U.K.
Abstract:
The most probable initial reaction between .NO and O2 is direct addition to give the peroxyl radical ONOO.. In view of the potential importance of this radical in biology, we have searched extensively for its formation, using EPR spectroscopy and rapid freezing techniques. At best, only extremely low concentrations were detected, and in most systems, no signals were detectable. We conclude that this radical is unlikely to be of major importance per se in biological systems, in contrast with its one electron adduct, the peroxynitrite anion.
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